Figure 5.miR-320 targeted CD36 promoter DNA in nucleus and leptin receptor mRNA in cytoplasm respectively. (A–E) Western blotting analysis of the effects of miR-320 on CD36 and LEPR rescued with nuclear or cytosol Ago2 re-expression. In Ago2 knockdown cells, re-expression of Ago2 in the cytoplasm restored miR-320-mediated LEPR suppression. While re-expression of Ago2 in the nucleus restored miR-320-mediated CD36 activation (n=3, *p<0.05).
