Figure 5. USP10 stabilizes CD36. (A, B) THP1 cells were treated with CHX or CHX+Spautin-1/USP10 siRNA for 0, 12, 24 and 36 h. CD36 expression was detected using western blot assay. (C) And then the band of CD36 was calculated. (D, E) Cells were treated with Spautin-1, MG132. Western blot was performed to test the expression of CD36. (F) Total RNA was extracted and RT-qPCR was performed to detected mRNA level of CD36. *p<0.05 versus Spautin-1 treatment.