Research Paper Volume 12, Issue 21 pp 21809—21836

Figure 6. PBRM1 silencing enhances tumor angiogenesis and promotes cell proliferation of RCC cells in vitro. (A) Matrigel tube formation assay results show the tube-like structures formed in the matrigel by CM from control and PBRM1-silenced 786-O and Caki-1 cells. (B) MTT assay results show viability of control and PBRM1-silenced 786-O and Caki-1 cells. (C) The colony formation assay results show the total numbers of colonies formed by control and PBRM1-silenced 786-O and Caki-1 cells based on crystal violet staining. (D) Flow cytometry analysis shows the percentage of G1, S, and G2-M cells in control and PBRM1-silenced 786-O and Caki-1 cells based on PI staining. Note: The experiments were performed in triplicate and data are represented as means±SD; the statistical analysis was performed using Student’s t-test.