Research Paper Volume 12, Issue 20 pp 20658—20683
Cross-talk between the ER pathway and the lncRNA MAFG-AS1/miR-339-5p/ CDK2 axis promotes progression of ER+ breast cancer and confers tamoxifen resistance
The MAFG-AS1/miR-339-5p/CDK2 axis regulates cell proliferation. (A) Expression of CDK2 and MAFG-AS1 were positively correlated. p=0.01, r=0.67. (B) Correlation between CDK2 and MAFG-AS1 in GEPIA. (C) Representative IHC expression of CDK2 and ki-67 shown from two patients with different MAFG-AS1 expressions. (D) Relative expression of MAFG-AS1, miR-339-5p and CDK2 in the same cell lines. (E) qRT-PCR analysis of the expression of CDK2 with over-expression of MAFG-AS1 and miR-339-5p in T47D and MCF-7 cells. (F) Western blot was performed to detect CDK2 expression in T47D and MCF-7 with MAFG-AS1 and miR-339-5p over expression.(G) The expression of CDK2, cyclin E, Rb, FOXO1, p-FOXO1, CDK4, CDK6 and CyclinD1 after MAFG-AS1 knockdown compared with NC in T47D and MCF-7 cells. (H) The expression of PARP, TRAIL, cl-caspase3, cl-caspase8 and cl-caspase9 after MAFG-AS1 knockdown compared with NC in T47D and MCF-7 cells. (I) Correlation between FOXO1 and MAFG-AS1 at mRNA level in GEPIA.