Figure 1. Characterization and identification of HMSC-27b-EV. (A) TEM imaging of HMSC-27b-EV, 5 parallel samples were analyzed; (B, C) The size distribution and Zeta potential of HMSC-27b-EV measured by DLS, 5 parallel samples were analyzed; (D) The detection of CD9 and CD63 in exosomes by western blot; (E) The levels of CD9 and CD63 in HMSC-27b-EV that detected by Flow cytometry; (F) miRNA-27b concentration in HMSC-27b-EV detected by RT-PCR, and HMSC • EV@nHAC was set as the control group. n = 5, *p < 0.05 as determined by Student's t-test.