Research Paper Volume 12, Issue 21 pp 21446—21468

High phosphate induces skeletal muscle atrophy and suppresses myogenic differentiation by increasing oxidative stress and activating Nrf2 signaling

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Figure 6. Inhibition of Pi transport, ROS production, or Nrf2 activity counteracts high Pi-induced changes in Nrf2, p62, and myogenin expression in differentiated C2C12 cells. (A, B) Representative immunoblots of p62, Nrf2, and myogenin expression in whole-cell lysates of 3-day-differentiated C2C12 cells treated for 24 h with (A) PFA or (B) 10 μM Mito-TEMPO or 10 mM NAC. (C) Immunoblot analysis of Nrf2, p62, and myogenin expression in whole-cell lysates of 3-day-differentiated C2C12 cells treated for 24 h with 4 mM Pi and various Nrf2 modulators. β-actin was used as loading control. Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding control Pi (-) plus vehicle, Mito-TEMPO, or NAC. #P > 0.05.