Research Paper Volume 12, Issue 18 pp 18588—18602

Figure 1. Upregulation of miR-132/-212 in postoperative HAEC patients or enteritis animal models. (A) The expression of miR-132/212 was validated in dilated segments of 32 po-HAEC and 32 No-HAEC patients using qRT-PCR. MiR-132 and miR-212 expression were significantly increased in po-HAEC tissues comparing to No-HAEC samples. (B) qRT-PCR was applied to detect miR-132 and miR-212 expression in LPS-stimulated mice and control groups (n=6 each). (C) The pathological changes in mice challenged with LPS was observed by HE staining. Scale bar: 50 μM. (D) Mean serum concentrations of IL-18 and IL-1β in LPS-stimulated mice and control groups was evaluated using ELISA assay. Data were expressed as mean ± SD. *P<0.05, ** P<0.01. (E) qRT-PCR analysis was applied to detect dose- and time-dependent expression of miR-132 and miR-212 in HT29 cell line treated with 0-1000 ng/ml LPS for 4-24 h.