Research Paper

Figure 2. Silencing lncRNA CASC15 reduces the proliferation and invasion of ESCC cells. (A) RT-qPCR to detect the silencing efficiency of lncRNA CASC15. (B) CCK-8 assay to assess the viability of KYSE270 and EC109 cells in response to silencing lncRNA CASC15. (C) Transwell assay to evaluate the invasion of KYSE270 and EC109 cells after silencing lncRNA CASC15 (× 200). (D–E) Western blot analysis to determine the protein level of Ki-67 (a proliferation-related factor) and MMP2 (an invasion-related factor) in KYSE270 and EC109 cells. *p < 0.05 vs. the ESCC cells transfected with sh-NC. The measurement data were presented as the mean ± standard deviation. Data in panel (A, C, E) were analyzed by unpaired t test and those in panel (B) by one-way analysis of variance, followed by Tukey’s post hoc test. the experiment was repeated 3 times. sh-NC, cells transfected with negative control plasmids of short hairpin RNA; sh-CASC15, cells transfected with short hairpin RNA against lncRNA CASC15.