Research Paper

Figure 1. LncRNA CASC15 is overexpressed with poor prognostic value in ESCC. (A) Analysis of five aberrant lncRNAs in ESCC-related gene expression dataset GSE45168 in GEO database. (B) The expression of five aberrant lncRNAs in GSE45168 (n = 5. the left box plot represents the expression of lncRNAs in the normal sample, and the right box plot represents the expression of lncRNAs in the ESCC samples). (C) The expression of lncRNA CASC15 in ESCC and adjacent normal tissues analyzed in the TCGA database. (D) The expression of lncRNA CASC15 in EC109 cells detected by different primers. (E) The expression of lncRNA CASC15 in ESCC and adjacent normal tissues (n = 79 including 6 cases in stage I, 31 in stage II, 27 in stage III, and 15 in stage IV) determined by RT-qPCR (*p < 0.05 vs. the adjacent normal tissues). (F) The expression of lncRNA CASC15 in ESCC cell lines and HEEC cells determined by RT-qPCR (*p < 0.05 vs. the HEEC cell line). (G) Kaplan-Meier survival curve to analyze the correlation between lncRNA CASC15 expression and overall survival rate of 79 ESCC patients (log-rank test for the difference significance). The measurement data in panel (D–F) were presented as mean ± standard deviation and compared with one-way analysis of variance, followed by Tukey’s post hoc test.