Research Paper Volume 12, Issue 23 pp 23668—23683

Figure 6. Effects of DNMT3B knockdown on the protein levels of HNF4γ and Notch1, which are downstream targets of miR-34a. (A) miR-34a has binding sites on the 3’ UTR of HNF4γ and Notch1 mRNAs. (B) The protein levels of HNF4γ and Notch1 in EJ and UMUC3 cells transfected with miR-NC, miR-34a mimic, or the miR-34a inhibitor (inhibitor). (C) The protein levels of HNF4γ and Notch1 in DNMT3B-knockdown EJ and UMUC3 cells after being infected with recombinant lentiviral vectors containing shRNAs targeting DNMT3B (shRNA) and negative control cells infected with empty lentivirus (NC). (D) The protein levels of HNF4γ and Notch1 in the shRNA groups of EJ and UMUC3 cells transfected with the miR-34a inhibitor (shRNA+inhibitor) or miR-NC (shRNA+miR-NC). NC cells were used as the control. DNMT3B, DNA methyltransferase 3B; HNF4γ, hepatocyte nuclear factor 4 gamma; UTR, untranslated region; NC, negative control; shRNA, short hairpin RNA.