Research Paper Volume 12, Issue 19 pp 19365—19374

Knockdown of NHP2 promotes apoptosis of PLC/PRF5 hepatoma cells stably overexpressing HBx or not. (A) Representative flow cytometry assay of PLC/PRF5 cells with or without HBx promotion in the absence or presence of NHP2 silencing. (B) Quantitative analysis for cell apoptosis from flow cytometry assay. (C) Representative western blot bands together with quantitative analysis for Bcl-2, Bax and cleaved-Caspase 3 expression of PLC/PRF5 cells in the aforementioned groups. Ov: overexpression, NC: negative control, HBX: Hepatitis B virus X. ^*P<0.05, ^**P<0.01 and ^***P<0.001.