circPDZD8 was upregulated in gastric cancer tissues and cell lines. (A) circPDZD8 expression was detected in gastric cancer tissues and normal tissues by RT-qPCR (n=20). (B) circPDZD8 was upregulated in the different gastric cancer cells. (C) circPDZD8 was enriched in MGC-803 cytoplasm fraction. Levels of circPDZD8, GAPDH and U6 RNA in purified MGC-803 nuclear and cytoplasm fractions were detected by RT-qPCR; *** P < 0.001.