Research Paper Volume 12, Issue 20 pp 20285—20307

Figure 7. DGCR5/miR-23a axis affect glioma cell proliferation and apoptosis through PTEN. (A) U251-MG and SHG44 cells were transfected with lncRNA DGCR5-overexpressing vector and examined for the protein levels of PTEN by immunoblotting. Next, U251-MG and SHG44 cells were co-transfected with DGCR5 and miR-23a mimic, (B) DNA synthesis capacity was determined by EdU assay (C); cell apoptosis was determined by Flow cytometry assay; (D) the protein levels of PTEN, p-AKT, AKT, p-PI3K, PI3K, and Cyclin D1 were determined by immunoblotting. *P<0.05, **P<0.01, compared to the control group; #P<0.05, ##P<0.01, compared to the vector + miR-23a mimic group.