Research Paper Volume 15, Issue 2 pp 583—594

Figure 4. Ectopic overexpression of Lnc-EPIC1 promotes colon cancer cell progression in vitro. The stable human colon cancer cells pri-Can-1/-2/-3, with empty vector (“Vec”) or the Lnc-EPIC1-expression construct (“OE-Lnc-EPIC1-Line1/2”, indicating two stable lines) were cultured for applied time periods, expression of Lnc-EPIC1 and linear EPIC1 was tested by qPCR assays (A, B, H); Cell viability was examined by CCK-8 assays (C, I); Cell growth and proliferation were examined by cell counting assay (D) and nuclear EdU staining (E, J), respectively. Cell migration and invasion were tested by “Transwell” assay (F) and “Matrigel Transwell” assay (G), respectively. Results were quantified. Data presented as mean ± standard deviation (SD). * p< 0.05 vs. “Vec” cells. Experiments in this figure were repeated five times.