Research Paper Volume 12, Issue 18 pp 18436—18452

Figure 6. Nudt21 is induced by H₂O₂ and down-regulates HMGA2 in hTSCs. (A) hTSCs were subjected to H₂O₂ treatment at indicated concentrations for 24 h. The protein levels of Nudt21 were determined by western blot analysis. n = 3; *p < 0.05, **p < 0.01 vs. 0 mM H₂O₂. (B) hTSCs transfected with shNudt21-1, shNudt21-2 or a scrambled control shRNA (nc) lentivirus were cultured in the presence or absence of 0.5 mM H₂O₂ for 24 h. Nudt21 and HMGA2 protein levels were determined by western blot analysis. Untransfected cells (mock) were included for comparison. (C–E) hTSCs transfected with a scrambled control shRNA (nc), shNudt21-2 and shHMGA2, alone or in combination as indicated were cultured in the presence or absence of 0.5 mM H₂O₂ for 24 h. (C) Clonogenicity was evaluated by the colony formation assay. (D) Cell viability was determined by the CCK-8 assay. (E) Cell senescence was assessed by β-gal staining 72 h after H₂O₂ treatment. The data shown are from three replicates and are indicated as mean ± SD. *p < 0.05, **p < 0.01.