Research Paper Volume 12, Issue 19 pp 19233—19253
CircRNA CDR1as promotes hepatoblastoma proliferation and stemness by acting as a miR-7-5p sponge to upregulate KLF4 expression
MiR-7-5p attenuates the function of CDR1as in HB cells. (A, B) The colony formation assay and EdU assay indicated that transfected with si-CDR1as could decrease the cell proliferative ability in HepG2 and HUH6 cells, and the co-transfection of si-CDR1as and miR-7-5p inhibitors abolished the si-CDR1as-induced attenuation of cell proliferation; (C) The sphere-forming assay indicated that transfected with si-CDR1as could decrease the sphere-forming capacity in HepG2 and HUH6 cells, and the co-transfection of si-CDR1as and miR-7-5p inhibitors abolished the si-CDR1as-induced attenuation of sphere-forming capacity; (D) The flow cytometric analysis indicated that transfected with si-CDR1as could decrease the proportion of CD133+ cells in HepG2 and HUH6 cells, and the co-transfection of si-CDR1as and miR-7-5p inhibitors abolished the si-CDR1as-induced decrease of CD133+ cells rate. Scale bar, 200 μm. Data are presented as the mean ± SEM of three experiments. *P < 0.05,**P < 0.01,***P < 0.001 (Student’s t-test).