Research Paper Volume 12, Issue 14 pp 13958—13978

Figure 3. Seahorse extracellular flux analysis for metabolic potential and glycolytic flux in senescent AML12 cells. Agilent Seahorse XF Cell Energy Phenotype Test kit was used with Agilent Seahorse XFe96 Extracellular Flux Analyzer to analyze control and senescent AML12 cell’s metabolic potential (A) and stressed phenotype (B). Oxygen consumption rate (OCR) represents mitochondrial respiration while the extracellular acidification rate (ECAR) represents glycolytic potential under basal and stressed conditions (as described in Methods) (A). Percent change in mitochondrial oxidative phenotype (OCR) and glycolytic phenotype (ECAR) under stress over basal (100%) conditions (B). Agilent Seahorse XF Glycolysis Stress Test kit was used with Agilent Seahorse XFe96 Extracellular Flux Analyzer to analyze control and senescent AML12 cell’s glycolytic flux and reserve capacity (C). Glycolytic metabolic parameters were calculated as described in the Methods (D). All the parameters presented in the panel B and D were calculated using Seahorse Wave Desktop software. Statistical differences were calculated significant as **p<0.01; ****p<0.0001.