Research Paper Volume 12, Issue 20 pp 20152—20162
Polymerase I and transcript release factor transgenic mice show impaired function of hematopoietic stem cells
PTRF expression increased during aging and skewed differentiation potential of HSCs. (A) PTRF mRNA levels in the bone marrow of young (2-mo) and old mice (20-mo), n=3. (B) Immunoblot showing PTRF protein expression in the bone marrow (BM) of young and old mice, n=3. (C) PTRF mRNA levels in the BM of 2-month old PTRF transgenic and wild-type (WT) mice, n=3. (D) Immunoblot showing PTRF protein expression in the BM of young PTRF and WT mice, n=3. (E–G) The percentage of B cells (E), M cells (F) and T cells (G) in the peripheral blood, BM and spleen of young PTRF transgenic mice. Data represent the mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01. Data were normalized against GAPDH expression.