Research Paper Volume 12, Issue 18 pp 18384—18395

Figure 4. p38γ KO inhibits human OS cell progression in vitro. Expression of listed genes in the stable monoclonal OS1 cells, with the empty vector (“Cas9-C”) or the applied lenti-CRISPR/Cas9-p38γ-KO construct (with verified sgRNA, “sgRNA-1/-2”) was tested by qPCR and Western blotting assays (A, B). Cells were further cultured for applied time periods, cell proliferation (by measuring EdU ratio, C), migration (“Transwell” assay, D), invasion (“Matrigel Transwell” assay, E) and apoptosis (by measuring nuclear TUNEL ratio, F) were tested, and results quantified. Expression of listed proteins was quantified and normalized to the loading control (B). Data presented as mean ± standard deviation (SD, n=5). *** p< 0.001 vs. “Cas9-C” control cells. Experiments in this figure were repeated five times. Bar=100 μm (C–F).