Figure 5. Housekeeping gene expression in the hypothalamus. (A) Proportion of housekeeping genes in differentially expressed genes (p value < 0.05). (B) The overlap of differentially expressed housekeeping genes according to age and gender. (C) The heatmap shows an example of stably expressed housekeeping genes. (D–G) The heatmap represents the differentially expressed housekeeping genes of middle-aged females vs. males (D), young-aged females vs. males (E), middle-aged vs. young-aged males (F), and middle-aged vs. young-aged females (G). (H) The protein interactions of differentially expressed housekeeping genes of middle-aged vs. young-aged females. (I) The KEGG pathway enrichment and GO biological process of differentially expressed housekeeping genes of middle-aged vs. young-aged females.