Research Paper
Exosomal miR-211 derived from cancer stem cells promotes cancer progression by activating autophagy in NSCLC
Figure 1. CSCs secreted exosomal miR-211 in NSCLC. (A) Immunofluorescence staining for CD105 and Netin expression of CSCs 3D sphere. DAPI indicates nucleus. Scale bar = 20 μM. (B) qRT-PCR analyzed the expression of miR-211 in TCs and CSCs. n=6, *p<0.05. (C) TEM of exosomes isolated from TC and CSC. Scale bar = 500 nM. (D) Western blot for CD63, Tsg101 and Alix in exosomes. (E) The expression of miR-211 in exosomes was tested by qRT-PCR. n=6, *p<0.05.