Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 20 pp 20139—20151
Circular RNA circ_PVT1 induces epithelial-mesenchymal transition to promote metastasis of cervical cancer
Circ_PVT1 binds directly to miR-1286 in C33A cells. (A) Schematic for predicting miR-1286 binding sites of circ_PVT1 (https://circinteractome.nia.nih.gov/api/v2/mirnasearch?circular_rna_query=hsa_circ_0009143&mirna_query=hsa-miR-1286&submit=miRNA+Target+Search). (B) The luciferase assay was performed to verify the interaction between miR-1286 and circ_PVT1. n=5, *P<0.05. (C) Binding situation between circ_PVT1 and miR-1286 verified using RNA pull-down. (D) The enrichment of circ_PVT1 was detected by RIP assay. n=5, **P<0.01. (E) Co-localization between circ_PVT1 and miR-1286 was revealed by fluorescence in situ hybridization. (F) The expression of miR-1286 were assessed in C33A cells. n=10, **P<0.01.