Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 18 pp 18084—18098
RAI14 silencing suppresses progression of esophageal cancer via the STAT3 pathway
Figure 6. Regulation of RAI14 in EC proliferation depends on the STAT3 pathway. The STAT3 inhibitor, AG490, was dissolved in DMSO. TE-14 cells that were transfected with the RAI14-vector or RAI14 were treated with DMSO (Vehicle) or AG490. (A) Protein levels of RAI14, p-STAT3, total STAT3, Mcl-1, cyclin D1, and cleaved caspase-3 were determined by western blotting. GAPDH was used as the internal control. (B) The proliferation of cells was assessed using the CCK-8 assay. (C) Flow cytometry scatter plot of apoptosis. (D) Flow chart depicting cell cycle distribution that was assessed by flow cytometry. (E) Apoptosis rate. (F) Proportion of cells in each cell-cycle phase. *P < 0.05 versus the respective control group.