Research Paper Volume 12, Issue 18 pp 18073—18083

Figure 5. MiR-141-3p inhibited RIPK1-mediated necroptosis to reduce LPS-induced Caco-2 cell injury. Cell viability (A), PI positive staining (B), IL-6 mRNA level (C), TNF-α mRNA level (D) of LPS-treated Caco-2 cells with RIPK1 overexpression in the presence of miR-141-3p mimics. (E) The protein level of RIPK1, p-RIPK3, RIPK3, p-MLKL and MLKL in LPS-treated Caco-2 cells in the presence or absence of miR-141-3p mimics. (F) IP assay was carried out to detect the interaction between RIPK1 and RIPK3 in LPS-treated Caco-2 cells in the presence or absence of miR-141-3p mimics. Scale bar = 20 μm. ^*p<0.05, ^**p<0.01.