Research Paper Volume 12, Issue 15 pp 15566—15580
The association between KLF4 as a tumor suppressor and the prognosis of hepatocellular carcinoma after curative resection
Figure 4. KLF4 inhibits the proliferation and migration ability of HCC cells. (A) Knockdown of KLF4 in SMMC-7721 and overexpression of KLF4 in PLC/PRF/5 was verified by western blot, and β-actin was used as an internal control in western blot assays. (B) CCK8 assay was implemented to detect the proliferation rate of steadily transfected SMMC-7721 and PLC/PRF/5. (C) Cell wound scratch assay and (D) transwell assay was executed to evaluate the migration rate of steadily transfected SMMC-7721 and PLC/PRF/5. Student’s t-test was used in line charts and bar charts. P < 0.05 was considered statistically significant. * P < 0.05, ** P < 0.01, *** P < 0.001.