Research Paper Volume 12, Issue 18 pp 18033—18051

Figure 8. Increased glycogen synthesis associated with inactivation of GSK3α/β in liver and muscle of GHRH^-/- mice. (A) Increased glycogen synthesis in liver and muscle in GHRH^-/- mice. All data were represented as mean ± sem (n = 4-5 mice for each genotype). ** P < 0.01 versus vehicle within time points. (B–D) Activation of GSK3α/β in skeletal muscle. (E–G) Activation of GSK3α/β in liver. The 4 hours fasted mice were i.p. injected with porcine insulin (1 IU/kg of body weight). After 20 min, tissues were collected to perform western blots. All data were represented as the means ± sem (n=4 for WT group and n=6 for GHRH^-/- to detect activation of GKS3α/β). Statistical analysis was performed by unpaired Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.