Figure 6.Schematic of a genetic circuit in melanoma. (A) A gene circuit, with four modules was activated by two up-regulated transcription factors (RELA and STAT1), to express cholesterol 7-desaturase NVD-BM, and directly catalyzing cholesterol to 7-DHC, to repress melanoma growth and migration. (B) Gene circuit diagram constructed in melanoma cells based on CRISPR/Cas9 and Tet-ON systems.
