Research Paper Volume 12, Issue 14 pp 15021—15036
NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-ĸB signaling
Figure 5. The development of dual-input biosensors. (A) Up-regulated transcription factors in melanoma tissues compared with normal skin tissues, were filtered from the TCGA database. (B) A schematic diagram of transcription factors as input signals, activating biosensors using the luciferase assay. (C) Heatmap of luciferase expression levels using a single-input promoter in A375 and A2058 cells. (D) Heatmap of luciferase expression levels using dual-input promoters in A375 and A2058 cells. (***p < 0.001).