Figure 4.Heterogeneous cholesterol 7-desaturase NVD-BM converts cholesterol to 7-DHC. (A) GC/MS analysis of 7-DHC and cholesterol levels in A375 cells, transfected with NVD-BM and DAF-36, when compared with the negative control pCDH (NC), using 7-DHC and cholesterol standard curves. (B) 7-DHC concentrations in A375 cells, transfected with NVD-BM versus NC. (C) Cholesterol concentrations in A375 cells, transfected with NVD-BM versus NC. (*p < 0.05, **p < 0.01).
