Expression of Concern
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 13, Issue 10 pp 14456—14468
Cancer-associated fibroblasts secreted miR-103a-3p suppresses apoptosis and promotes cisplatin resistance in non-small cell lung cancer
Figure 5. Bak1 was a direct target of exosomal miR-103a-3p in NSCLC cells. (A) Predicted miR-103a-3p target sequences in the 3′ UTRs of Bak1 genes. (B) Bak1 mRNA expression in NCI-H1650 and NCI-H1299 transfected with miR-103a-3p or AMO- miR-103a-3p at 48 h after transfection. (C) Bak1 mRNA levels in NCI-H1650 and NCI-H1299 cells at 48 h after incubation with CAF exosomes. (D) WT and mutant Bak1 luciferase plasmids were transfected into HEK293 cells with miR-103a-3p or AMO- miR-103a-3p. The luciferase activity was measured by dual-luciferase reporter assay system. (E) The effects of CAF exosomes on Bak1 reporter luciferase activity in HEK293 cells. *p<0.05 vs miR-NC or AMO-miR-NC or NF exo.