Research Paper Volume 12, Issue 18 pp 17990—18007

Figure 6. Regulatory effects of SIRT1 on M2 polarization in microglia. BV-2 microglia were incubated with CAY (5 μM) (A) or minocycline (20 μM) (B) for the indicated time periods. (C) IMG cells were transfected with wild-type SIRT1 for 24 h. The expression levels of Ym-1, ARG1, IL-4, and IL-13 were determined by real-time PCR analysis. (D) Cells were pretreated with CAY (5 μM) for 30 min, followed by stimulation with ATP (300 μM) or no stimulation for 24 h. Transmigration activities were examined in vitro using a transwell insert system. The transmigrated cells were visualized by phase-contrast imaging (right panel). Results are expressed as means ± SEM of three independent experiments; * p < 0.05 compared with the control group. # p < 0.05 compared with the only ATP- or only CAY-treated groups.