Research Paper Volume 12, Issue 18 pp 17976—17989

Figure 7. HUA stimulates the expression of thrombus-related factors and the adhesion of monocytes and platelets to HUVECs via NF-κB pathway activation. Normal HUVECs were treated DMSO, and HUA-treated HUVECs were treated with DMSO or p65 inhibitor. (A) p65 and phosphorylated p65 expression in HUVECs detected by western blot analysis normalized to β-actin. (B) Phosphorylated p65 expression in HUVECs detected by immunofluorescence (× 400). (C) NF-κB DNA activity in HUVECs detected by ELISA. (D) VCAM-1, ICAM-1, PAI-1, TF, and T-PA expression in HUVECs determined by RT-qPCR normalized to GAPDH. (E) VCAM-1, ICAM-1, PAI-1, TF, and T-PA expression in HUVECs determined by western blot analysis normalized to β-actin. (F) VCAM-1 level in HUVECs determined by ELISA. (G) ICAM-1 level in HUVECs determined by ELISA. (H) PAI-1 level in HUVECs determined by ELISA. (I) TF level in HUVECs determined by ELISA. (J) T-PA level in HUVECs determined by ELISA. (K) Adhesion of monocytes to HUVECs (× 200). * p < 0.05 vs. normal HUVECs treated with DMSO. # p < 0.05 vs. HUA HUVECs treated with DMSO. The measurement data were shown as mean ± standard deviation and compared by one-way analysis of variance, followed by Tukey's post hoc test. The cell experiment was repeated three times independently.