Research Paper Volume 12, Issue 18 pp 17976—17989
Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-κB pathway by upregulating let-7c
Figure 6. HUA contributes to activation of the NF-κB pathway by repressing let-7c-targeted MEF2C. Normal HUVECs were used as controls, and HUA-treated HUVECs were transfected or not transfected with oe-NC, oe-MEF2C, NC-inhibitor, let-7c-inhibitor, let-7c-inhibitor + si-NC, or let-7c-inhibitor + si-MEF2C. (A) p65 and phosphorylated p65 expression in HUVECs detected by western blot analysis normalized to β-actin. (B) NF-κB DNA activity in HUVECs detected by ELISA. (C) Phosphorylated p65 expression in HUVECs detected by immunofluorescence (× 400). * p < 0.05 vs. control HUVECs. # p < 0.05 vs. HUA HUVECs transfected with oe-NC. & p < 0.05 vs. HUA HUVECs transfected with NC-inhibitor. $ p < 0.05 vs. HUA HUVECs transfected with let-7c-inhibitor + si-NC. The measurement data were shown as mean ± standard deviation and compared by one-way analysis of variance, followed by Tukey's post hoc test. The cell experiment was repeated three times independently.