Research Paper Volume 12, Issue 18 pp 17976—17989

Figure 5. The expression of thrombus-related factors and the adhesion of monocytes and platelets to HUVECs are promoted by HUA via let-7c-downregulated MEF2C. (A) RT-qPCR analysis of MEF2C expression in HUVECs after treatment with HUA. (B) western blot analysis of MEF2C protein expression in HUVECs. (C) si-MEF2C-1 and si-MEF2C-2 detected by RT-qPCR normalized to GAPDH. HUA-treated HUVECs were transfected with oe-NC, oe-MEF2C, let-7c-inhibitor + si-NC or let-7c-inhibitor + si-MEF2C. (D) Let-7c expression in HUVECs detected by RT-qPCR normalized to U6. (E) Protein expression of MEF2C in HUVECs detected by western blot analysis normalized to β-actin. (F) VCAM-1, ICAM-1, PAI-1, TF and T-PA expression in HUVECs determined by RT-qPCR normalized to GAPDH. (G) VCAM-1, ICAM-1, PAI-1, TF and T-PA expression in HUVECs determined by western blot analysis normalized to β-actin. (H) VCAM-1 level in HUVECs determined by ELISA. (I) ICAM-1 level in HUVECs determined by ELISA. (J) PAI-1 level in HUVECs determined by ELISA. (K) TF level in HUVECs determined by ELISA. (L) T-PA level in HUVECs determined by ELISA. (M) Adhesion of monocytes to HUVECs (× 200). * p < 0.05 vs. HUA HUVECs transfected with oe-NC. # p < 0.05 vs. HUA HUVECs transfected with let-7c-inhibitor + si-NC. The measurement data were shown as mean ± standard deviation and compared by one-way analysis of variance, followed by Tukey's post hoc test. The cell experiment was repeated three times independently.