Research Paper Volume 12, Issue 18 pp 17976—17989
Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-κB pathway by upregulating let-7c
Figure 4. MEF2C is negatively targeted by let-7c. (A) The binding sites between let-7c and MEF2C predicted in targetscan. (B) The binding relationship between let-7c and MEF2C evaluated by dual luciferase reporter assay. * p < 0.05 vs. the treatment of NC-mimic. (C) Expression of let-7c and MEF2C in HUA-treated HUVECs after alteration of let-7c determined by RT-qPCR normalized to U6 and GAPDH. (D) Protein expression of MEF2C in HUA-treated HUVECs after alteration of let-7c determined by western blot analysis normalized to β-actin. * p < 0.05 vs. HUA HUVECs treated with NC-mimic. # p < 0.05 vs. HUA HUVECs treated with NC-inhibitor. * p < 0.05 vs. normal HUVECs. The measurement data were shown as mean ± standard deviation. Data between two groups were compared by unpaired t-test, while comparisons among multiple groups were performed using one-way analysis of variance, followed by Tukey's post hoc test. The cell experiment was repeated three times independently.