Research Paper Volume 12, Issue 14 pp 14754—14774

Figure 8. Depletion of DSCAM-AS1 and knockdown of DCTPP1 and QPRT together affected BC cell growth and invasion. Cell viability (A), apoptosis (B), migration (C), and invasion (D) assays were performed after silencing DSCAM-AS1 or DCTPP1 and QPRT together. DSCAM-AS1 depletion and knockdown of DCTPP1 and QPRT remarkably inhibited cell viability, migration, and invasion in MCF-7 and T47D cells; however, they promoted apoptosis. The bar in the pictures (D) indicates a length of 5 μm. (E) In the in vivo assay, silencing DSCAM-AS1 or DCTPP1 and QPRT together dramatically inhibited MCF-7 and T47D cell growth in nude mice. (F) The molecular mechanisms behind DSCAM-AS1 regulating DCTPP1 and QPRT. DSCAM-AS1 increased QPRT expression through sponging miRNA-150-5p and miRNA-2467-3p. In contrast, DSCAM-AS1 promoted the transcription of the DCTPP1 gene by affecting H3K27 acetylation and enhancing DCTPP1 mRNA stability through binding to the 3’UTR, which collectively resulted in the overexpression of DCTPP1. **P<0.01 and ***P<0.001 vs. control group. DSC(KD): DSCAM-AS1 knockdown; DCT(KD):DCTPP1 knockdown; QPR(KD): QPRT knockdown.