Research Paper Volume 12, Issue 14 pp 14754—14774
Silencing DSCAM-AS1 suppresses the growth and invasion of ER-positive breast cancer cells by downregulating both DCTPP1 and QPRT
Figure 2. The regulatory effects of DCTPP1 and QPRT on BC cell growth and apoptosis. DCTPP1 and QPRT mRNA (A) and protein (B) levels in MCF-7 and T47D cells were changed after transfection with shRNA-DCTPP1, shRNA-QPRT, and DCTPP1 and QPRT expression vectors. (C) Down-regulation of DCTPP1 or QPRT was associated with reduced viability in both MCF-7 and T47D cells and increased DCTPP1 or QPRT was associated with increased cell viability. (D) The apoptosis rate of MCF-7 and T47D cells increased after knocking down either DCTPP1 or QPRT. DCTPP1 overexpression decreased the apoptosis rate of MCF-7 and T47D cells, and QPRT overexpression only marginally decreased MCF-7 and T47D apoptosis. *P<0.05, **P<0.01 and ***P<0.001 vs. control group.