Figure 4.DMC-BH-induced apoptosis in GSCs is mediated by JNK/ERK signaling. (A) Phosphorylation protein levels in DMC-BH-treated GSCs analyzed by Proteome Profiler Human Phospho-Kinase Array. (B) Western analysis of phosphorylated levels of MAPK-related proteins p-ERK, p-JNK, p-c-Jun, and p-JunB in DMC-BH-treated GSCs.
(C) Caspase-3 activity measured after incubation with ERK inhibitor U0126 (20 μM) or JNK inhibitor SP600125 (30 μM). (D) Phosphorylated levels of MAPK-related proteins analyzed in DMC-BH-treated GSCs in the presence of U0126 (20 μM) or SP600125 (30 μM); n =3.
