Research Paper Volume 12, Issue 13 pp 13647—13667

Figure 7. SMARCA5 restoration reverses miR-146b-5p-mediated inhibition of F GSC/MSC proliferation and metastasis. (A) CCK8, (B and C) colony formation, and (D and E) EdU assays were conducted to evaluate F GSC/MSC proliferation after transfection with miR-146b-5p alone or co-transfection with miR-146b-5p plus SMARCA5. (F and G) Transwell assays to assess the invasiveness of F GSC/MSCs transfected with miR-146b-5p alone or with miR-146b-5p plus SMARCA5. (H and I) Wound healing assays to assess migration of F GSC/MSCs transfected with miR 146b 5p alone or with miR-146b-5p plus SMARCA5. (J) Xenograft model for evaluation of tumorigenesis in vivo. (K) Western blot analysis of proteins in the TGF β pathway in F GSC/MSCs transfected with miR-146b-5p alone or with both miR 146b 5p and SMARCA5.