Research Paper Volume 12, Issue 14 pp 14452—14466
Long noncoding RNA LINC01559 promotes pancreatic cancer progression by acting as a competing endogenous RNA of miR-1343-3p to upregulate RAF1 expression
Figure 2. Silence of LINC01559 expression suppressed PC cell proliferation in vivo and in vitro. (A) The effects of transfection in PC cell lines were detected by PCR assay. (B) CCK-8 assay was performed to test the cell viability and proliferation in control group (shCtrl) and silence groups (shlinc01559). (C, D) Colony formation assay was performed to test the cell colony ability in shCtrl and shlinc01559. (E) Tumor volume of the subcutaneous xenografts in shCtrl and shlinc01559. (F) Weight change curve. (G) IHC staining for LINC01559 and representative images of three pairs of subcutaneous xenograft tissue (100×).(bar: 100 μm) (H) The relative expression of Ki67 and PCNA in tumor tissue. *p < 0.05, **p < 0.01, ***p < 0.001