Research Paper Volume 12, Issue 14 pp 14391—14405

Figure 5. DDR1 promotes the EMT of HCC cells by regulating STAT3. siRNA of STAT3 or NC was transfected into Hep3B cells, the transfection efficiency was tested by qRT-PCR (A) and western blot (B) (n = 6, *p<0.05). DDR1 was cotransfected into Hep3B cells with si-STAT3. (C) CCK8 assay was used to calculate cell proliferation (n = 10 *p<0.05). (D) Wound healing assay was used to detect cell migration (n = 6, *p<0.05). (E) Transwell assay was performed to check cell invasive ability (n = 6, *p<0.05). qRT-PCR (F) and western blot (G) were used to test the expression of EMT related genes: Vimentin, N-cadherin and E-cadherin (n = 6, *p<0.05). (H) The protein expression of glutamine metabolism related genes: GLUD1, GLS1 and SLC1A was determined by western blot (n = 6, *p<0.05).