Research Paper Volume 12, Issue 14 pp 14391—14405

Figure 2. DDR1 promotes EMT and glutamine metabolism in Hep3B cells. DDR1 plasmid or NC was transfected into Hep3B cells. The expression of DDR1 was determined by qRT-PCR (A) and western bolt (B) (n = 6, *p<0.05). (C) CKK-8 assay was used to examine the cell growth at 0, 24, 48 and 72 h (n = 10,*p<0.05). (D) Wound healing assay was used to detect cell migration (n = 6, *p<0.05). (E) Transwell assay was performed to check cell invasive ability (n = 6, *p<0.05). qRT-PCR (F) and western blot (G) were used to test the expression of EMT related genes: Vimentin, N-cadherin and E-cadherin (n = 6, *p<0.05). The protein expression of glutamine metabolism related genes: GLUD1, GLS1 and SLC1A was determined by western blot (H) and immunofluorescence (I) (n = 6, *p<0.05).