Research Paper Volume 12, Issue 14 pp 14285—14299

Figure 4. C5aR deficiency or C5aR signaling blockade inhibits the downregualtion of p21 expression in tumor tissues of mice. Mice were received 4T-1 cells transplantation for tumor development and sacrificed in the indicated time. (C5aRa treatment group: WT mice were injected with C5aRa 30 min prior to 4T-1 cells transplantation. Saline was used as the control of C5aRa). The tumors tissues were collected. The expression of p21 and p-p21 was measured by western blotting (A) and immunohistochemistry (B, C). (D) The mRNA levels of p21 were measured by quantitative RT-PCR. (n=5-6/group). *P < 0.05, ***P < 0.001. GAPDH was used as the loading control. Magnification: 200×.