Research Paper Volume 12, Issue 13 pp 13463—13476

Figure 3. mtROS activated JNK signaling and stimulated SOD2 transcription in BPV-induced neuron oxidative stress. Con: untreated cells; NAC: cells treated with 5 mM NAC for 30 min; BPV: cells treated with 2.0 mM BPV for 60 min; NAC+BPV: cells pretreated with 5 mM NAC for 30 min, following treated with 2.0 mM BPV for 60 min. (A, B) the fluorescence intensities of mtROS in cells pretreated with NAC, following incubation with BPV; (C, D) the western blot analysis showed JNK phosphorylation in cells pretreated with NAC, following incubation with BPV; (E, F) the western blot analysis showed SOD2 transcription in cells pretreated with NAC, following incubation with BPV; (G) LDH production in cells treated with 2.0 mM BPV for 60min and/or pretreated with 5 mM NAC for 30min. Values are the mean± SEM of n = 3, *: P< 0.05.