This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 14 pp 14157—14173
Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS
Figure 2. Hsa_circ_0000073 induced proliferation, migration and invasion of OS cells in vitro. (A) Using hsa_circ_0000073 sequence information, shRNAs and hsa_circ_0000073 overexpression plasmids of were constructed. (B) The overexpression and knockdown effects in MG-63 and U2OS cells were analyzed by qRT-PCR. (C, D) Edu staining and CCK-8 assays were conducted to confirm the influences of hsa_circ_0000073 overexpression and knockdown on the proliferation of MG-63 and U2OS cells. Magnification, ×100; Scale bar=100 μm. (E, F) Transwell assays were adopted to assess the changes in migration and invasion abilities in hsa_circ_0000073-overexpressing and hsa_circ_0000073-silenced MG-63 and U2OS cells. Magnification, ×100; Scale bar=100 μm. *P<0.05.