Research Paper Volume 12, Issue 14 pp 14141—14156
Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2
Figure 3. AC092171.4 overexpression increases proliferation, invasion and migration in HCC cell lines. (A) QRT-PCR analysis shows AC092171.4 levels in the control and AC092171.4 overexpressing PLC/PRF/5 and Hep3B cells. (B) CCK-8 assay results show cell proliferation status of control and AC092171.4 overexpressing PLC/PRF/5 and Hep3B cells. (C) EdU assay results show proliferation status of control and AC092171.4 overexpressing PLC/PRF/5 cells. (D) EdU assay results show proliferation status of control and AC092171.4 overexpressing Hep3B cells. (E) Colony formation assay results show the total number of colonies in control and AC092171.4 overexpressing PLC/PRF/5 cells. (F) Colony formation assay results show the total number of colonies in control and AC092171.4 overexpressing Hep3B cells. (G and H) Transwell migration assay results show the total numbers of migrating control and AC092171.4 overexpressing PLC/PRF/5 and Hep3B cells. (I and J) Transwell invasion assay results show the total numbers of invasive control and AC092171.4 overexpressing PLC/PRF/5 and Hep3B cells. (K) Representative western blot shows the expression of E-cadherin (epithelial marker) as well as N-cadherin and vimentin (mesenchymal markers) in control and AC092171.4 overexpressing PLC/PRF/5 and Hep3B cells. * denotes p<0.05.