Research Paper Volume 12, Issue 14 pp 14141—14156

Figure 2. AC092171.4 silencing decreases proliferation, migration and invasion in HCC cell lines. (A) QRT-PCR analysis shows AC092171.4 expression in the sh-NC and sh-AC092171.4 transfected Huh7 and LM3 cells. (B) CCK-8 assay results show proliferation status of the sh-NC and sh-AC092171.4 transfected Huh7 and LM3 cells. (C) EdU assay results show proliferation status of the sh-NC and sh-AC092171.4 transfected Huh7 cells. (D) Colony formation assay results show the total number of colonies in the sh-NC and sh-AC092171.4 transfected Huh7 cells. (E) EdU assay results show proliferation status of the sh-NC and sh-AC092171.4 transfected LM3 cells. (F) Colony formation assay results show the total number of colonies in the sh-NC and sh-AC092171.4 transfected LM3 cells. (G) Transwell migration assay results show AC092171.4 downregulation inhibited cell migration and invasion after transfection. (H) Transwell invasion assay results show the numbers of invasive sh-NC and sh-AC092171.4 transfected Huh7 and LM3 cells. (I) Representative western blot shows the expression of E-cadherin (epithelial marker) as well as N-cadherin and vimentin (mesenchymal markers) in the sh-NC and sh-AC092171.4 transfected Huh7 and LM3 cells. β-actin was used as loading control. * denotes p<0.05.