Figure 4. Liver adiposity and fibrosis are reduced in old mice after a single procedure of neutral blood exchange. Histological analysis (Oil Red-O and Masson’s trichome staining) of 10 μm liver sections from uninjured mice collected at 6 days after NBE. (A) Representative images of lipid droplets (fat) stained with Oil Red-O and Collagen (fibrosis) stained blue with Masson’s trichome show that NBE visibly reduced fat and fibrosis of old livers. (B) Adiposity Index (red pixels per section) and (C). Fibrotic index (numbers of fibrotic clusters per section) were determined as in [