Research Paper Volume 12, Issue 18 pp 17921—17929
circKIF4A sponges miR-127 to promote ovarian cancer progression
Figure 4. circKIF4A acts as a ceRNA to regulate JAM3. (A) The predicted binding sites of miR-127 within the JAM3 3’UTR. (B) Cells were transfected and luciferase assay was performed. (C) Cells were transfected and JAM3 expression was detected by qRT-PCR (left) and western blotting (right). (D) RIP assay showing the enrichment of circKIF4A, JAM3 and miR-127 on Ago2. (E) Cells were transfected and a RIP assay on Ago2 was performed. (F) Cells were transfected and JAM3 expression was detected by qRT-PCR (up) and western blotting (below). **P < 0.01.