Figure 4. Nrf2 activation contributes to ferroptosis resistance. The cells were transfected with lentiviruses (A and F). Western blot analysis of the level of Nrf2, HO-1, and SLC7A11 protein expression in each group and the representative quantification of these proteins. (B and G) Cell viability was determined by a CCK-8 assay (n = 3). (C and H) Western blot analysis of the level of GPX4 protein expression in each group and the representative quantification of GPX4. (D and I) The level of lipid peroxide MDA in each group. (E and J) The level of GSH in each group. The error bars represent the standard error from three replicates. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.